Neutral Protease

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Product Details:

Best Before 24 months from date of manufacture
Shelf Life 24 months
Chemical Composition Neutral protease, activator salts
Flavor Odorless, tasteless
Medicine Type Biological enzyme preparation
Efficacy Highly effective for cell dissociation and tissue digestion in biological research applications
Ph Level Optimal activity at pH 7.0
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Product Details
Product Description

Neutral Protease Product Specifications

Packaging Type Sealed HDPE bottle
Dosage Guidelines Dilute with buffer as recommended, avoid exposure to extreme pH
Ph Level Optimal activity at pH 7.0
Purity 99%
Effect Efficient tissue dissociation with preserved cell viability
Strength Activity 1.5 U/mg
Packaging Size 100g, 500g, 1kg, bulk options
Ingredients Neutral Protease (Dispase)
Function Enzymatic hydrolysis of proteins under neutral conditions
Dosage Form Powder
Shelf Life 24 months
Storage Instructions Store at 2-8C, protect from moisture and contamination
Dosage As per protocol requirements, commonly 0.5-2 mg/ml
Best Before 24 months from date of manufacture
Flavor Odorless, tasteless
Chemical Composition Neutral protease, activator salts
Medicine Type Biological enzyme preparation
Efficacy Highly effective for cell dissociation and tissue digestion in biological research applications

Handling Precautions Avoid inhalation and contact with eyes
Solubility Soluble in aqueous buffer solutions
Microbial Purity Absent of E. coli, Salmonella, and S. aureus
Endotoxin Level <10 EU/mg
Applications Cell isolation, cell culture, tissue dissociation, molecular biology research
Enzyme Source Bacillus polymyxa
CAS Number 37288-86-1
Transport Requirements Ambient temperature, avoid humidity
Safety Information For research and laboratory use only
Activity Unit Measured in units per mg (U/mg)
Molecular Weight Approximately 35 kDa
Color White or off-white
Stability Stable for 24 months under recommended conditions
Allergen Information Does not contain known allergens
Form Lyophilized powder
Moisture Content 5%
Irritancy Non-irritating
Expiration Date Indicated on packaging

Product Description

Neutral Protease offered by us is a Metallo, a neutral protease that is found in non-mammalian animals. Because of its moderate proteolytic action, the enzyme is particularly well suited for the production of primary cells and secondary cell culture. This protease is widely utilized with collagenase as a secondary enzyme in cell isolation and tissue dissociation applications. Neutral protease provides the benefits of a fast reaction rate, little industrial pollution, and a wide range of reaction conditions adaptability.

Efficient Cell Dissociation

Neutral Protease guarantees rapid and homogeneous tissue digestion, vital for preparing cell suspensions in research. Cells remain viable with minimal damage, supporting reliable downstream analysis and culture. Its optimized activity at neutral pH also ensures gentle processing without compromising cellular integrity.

Safe and Pure Enzyme Preparation

Manufactured under stringent controls, this enzyme is absent of E. coli, Salmonella, and S. aureus, making it ideal for sensitive biological applications. With a purity of 99% and endotoxin levels below 10 EU/mg, contamination risks are minimized, supporting consistent research outcomes and safety during handling.

Convenient Packaging and Storage

Available in sealed HDPE bottles in quantities ranging from 100g to bulk, Neutral Protease is easy to store and transport at ambient temperature. Clear labeling of expiration dates, accompanied by storage instructions, ensures maximum product stability and efficacy over a 24-month shelf life.

FAQ's of Neutral Protease:

Q: How should Neutral Protease be handled during laboratory procedures?

A: When handling Neutral Protease, avoid inhalation and any contact with eyes. Use appropriate protective gear and work in a well-ventilated area. Dilute the powder with buffer as recommended in your protocol and avoid exposure to extreme pH to maintain enzymatic activity.

Q: What is the recommended dose for Neutral Protease in tissue dissociation applications?

A: Dosage typically ranges from 0.5 to 2 mg/ml, depending on tissue type and protocol specifications. It should be diluted with an aqueous buffer solution, ensuring optimal pH around 7.0 for maximal enzymatic activity and consistent cell yield.

Q: Where should Neutral Protease be stored to maintain its stability?

A: Store Neutral Protease at 2-8C in its sealed HDPE bottle, away from moisture and contaminants. Proper storage preserves enzyme activity for up to 24 months from the manufacturing date, as indicated on the packaging.

Q: What are the benefits of using Neutral Protease from Bacillus polymyxa in research?

A: Neutral Protease efficiently hydrolyzes proteins under neutral conditions, achieving effective tissue dissociation while maintaining cell viability. Its high purity and microbial safety profile make it a reliable choice for cell isolation, cell culture, and molecular biology workflows.

Q: Is Neutral Protease safe for use in sensitive cell-based experiments?

A: Yes, Neutral Protease is non-irritating, does not contain known allergens, and is free from hazardous microbial contamination. Its low endotoxin levels further enhance safety, making it suitable for sensitive biological studies and cell culture applications.

Q: Can Neutral Protease be shipped and stored at ambient temperatures?

A: Neutral Protease can be transported at ambient temperature, provided it is protected from humidity. Upon receipt, it should be promptly stored at 2-8C to ensure long-term stability and efficacy.

Q: What applications is Neutral Protease best suited for in the laboratory?

A: Neutral Protease is ideal for cell isolation, tissue dissociation, cell culture preparation, and molecular biology research, providing efficient enzymatic hydrolysis of proteins under neutral pH conditions while preserving cell structure and viability.

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